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Human Andrology
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Elsamanoudy, A., Shaalan, D., Abo El-khair, S., gaballah, M., State, A., Helaly, A. (2017). NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram. Human Andrology, 7(4), 127-135. doi: 10.21608/ha.2017.1628.1014
Ayman Elsamanoudy; Dalia Shaalan; Salwa M. Abo El-khair; mohammad gaballah; Ahmed F. State; Ahmed M.N. Helaly. "NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram". Human Andrology, 7, 4, 2017, 127-135. doi: 10.21608/ha.2017.1628.1014
Elsamanoudy, A., Shaalan, D., Abo El-khair, S., gaballah, M., State, A., Helaly, A. (2017). 'NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram', Human Andrology, 7(4), pp. 127-135. doi: 10.21608/ha.2017.1628.1014
Elsamanoudy, A., Shaalan, D., Abo El-khair, S., gaballah, M., State, A., Helaly, A. NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram. Human Andrology, 2017; 7(4): 127-135. doi: 10.21608/ha.2017.1628.1014

NRF2 gene expression and DNA fragmentation markers as possible predictors of chronic smoking induced spermatozoa dysfunction in infertility with normal seminogram

Article 3, Volume 7, Issue 4, December 2017, Page 127-135  XML PDF (479.12 K)
Document Type: Original Article
DOI: 10.21608/ha.2017.1628.1014
Authors
Ayman Elsamanoudy1; Dalia Shaalan2; Salwa M. Abo El-khair2; mohammad gaballah email 3; Ahmed F. State4; Ahmed M.N. Helaly5
1Medical Biochemistry and Molecular Biology department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Clinical
2Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Mansoura University,Mansoura, Egypt
3dermatology, andrology and STDs department mansoura faculty of medicine mansoura Egypt
4Dermatology, Andrology & STDs Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
5Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Mansoura University
Abstract
Introduction: Male factor is responsible for about half of infertility problems. However, the reasons for the decrease in male fertility are still broadly unclear. The mechanisms of how smoking may impact male fertility have not been established. However, with its influence on different semen parameters, it is regarded as a risk factor for infertility.
Aim: To investigate the effect of chronic smoking on spermatozoa NRF2 expression and DNA fragmentation in infertile men with apparently normal seminogram and to determine if NRF2 expression and DNA fragmentation markers could be possible predictors of the impact of chronic cigarette smoking on male fertility.
Methods: Semen samples were collected from 170 subjects; 65 nonsmokers (40 fertile and 25 infertile) and 105 smokers (25 fertile and 80 infertile). NRF2 gene expression, 8-OHdG and DNA fragmentation were assayed.
Results: There were significant increases in 8-OHdG and %DNA fragmentation with a significant decrease in NRF2 gene expression in infertile smokers. ROC curve analysis of spermatozoa NRF2 gene expression showed 95% sensitivity 93.3% specificity at cutoff value ≤0. 931 (p <0.0001) differentiating infertile smokers from controls. Moreover, seminal 8-OHdG assay shows 93.4% sensitivity, 89.2% % specificity, at cutoff values >19.33 pg/ml predicting the detrimental effect of smoking on spermatozoa DNA.
Conclusion: Chronic cigarette smoking may be a hidden causative mechanism of delayed fertility. Spermatozoa NRF2 gene expression and seminal 8-OHdG levels may serve as sensitive diagnostic indicators predicting smoking induced infertility. So, the presence of normal seminal parameters could not be an exclusion of potential effect of chronic smoking on male fertility.
Keywords
Nrf2; DNA fragmentation; Male infertility; Smoking
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