Seminal clusterin level as a predictor for spermatogenesis before testicular sperm extraction

Kamal, Emad El Din; Hassan, Azza S.; Hosni, Amal; Badran, Aya

doi:10.21608/ha.2022.135466.1086

Seminal clusterin level as a predictor for spermatogenesis before testicular sperm extraction

Document Type : Original Article

Authors

¹ Andrology department, assiut university

² 1. Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Assiut University, Assiut, Egypt.

³ 2. Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.

⁴ Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Assiut University.

10.21608/ha.2022.135466.1086

Abstract

Background: A number of factors have been proposed to be of predictive benefit for males with a good probability to retrieve
sperms; as testicular volume, serum FSH, total testosterone and Inhibin B levels. However, a little information has been
present regarding the use of seminal Clusterin (CLU) for that purpose.
Aim: We designed the study in order to estimate seminal CLU level for non-obstructive azoospermia (NOA) males undergoing
Testicular sperm extraction (TESE), also to detect the possibility of its use as a predictor for spermatogenesis in those patients.
Further, we aimed to analyze its relation to testicular size, semen volume, presence or absence of varicocele, hormonal profile
and result of MD-TESE.
Patients and Methods: A cross-sectional hospital-based study on a total of 176 males (of them; 88 with NOA and 88 normal
fertile). A semen sample was obtained from each patient before micro-dissection (MD)-TESE surgery and from normal fertile
males. Seminal CLU was measured in all samples by Enzyme linked immunosorbent assay (ELISA).
Results: Seminal CLU level significantly differ between NOA patients and normal fertile males (P-value = 0.000*). A
significant positive relation was obtained between seminal CLU level with both semen volume and testicular size (P-value
< 0.05). A non-significant correlation was obtained between seminal CLU level and result of micro-TESE (P-value > 0.05).
Conclusion: CLU concentration in se minal plasma, which is proportional to the expression level of CLU in the testis differ
significantly between normal fertile males and NOA patients. Seminal CLU level not significantly associated with whether or
not spermatozoa can be successfully retrieved by MD-TESE. Thus, Seminal CLU level couldn't be used as a useful biomarker
for the assessment of spermatogenic status in NOA patients before MD-TESE

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