Document Type : Original Article
Andrology department, Faculty of Medicine, SUEZ CANAL UNIVERSITY - FOM
Genetics Unit, Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University
Dermatology Department, Elmenshawy General Hospital
Dermatology Department Port said General Hospital
Dermatology Department El Arish General Hospital
lecturer of andrology, faculty of medicine suez canal university, Ismalia, egypt
Background: MicroRNAs (miRNAs) play essential roles in human spermatogenesis, but little is known about seminal
plasma miRNAs in infertile men.
Aim: To assess miRNA-34b, miRNA-181a, and miRNA-429 expression profiles in seminal plasma of patients with
nonobstructive azoospermia (NOA) and to correlate these expression profiles with their clinical, laboratory, and
Patients and Methods: This was a prospective case–control study which included two groups: 50 males with NOA and 50
healthy fertile males who attended the Andrology Outpatient Clinic, the Genetics Unit, and a private IVF Center. Semen
analysis and assessment of the three miRNAs expression profiles in seminal plasma were done by real-time PCR for both
groups. Hormonal profile assessment (follicle-stimulating hormone, luteinizing hormone, testosterone, and prolactin),
testicular sperm extraction, and histopathology were done for the patients’ group.
Results: In this study, there were statistically significant upregulations of both miRNA-429 and miRNA-181a in the patients’
group compared with controls (P<0.001), which was not the case for miRNA34b (P=0.259). There were statistically significant
relationships between the three miRNAs, testicular sperm extraction results, and histopathological patterns (P<0.05).There
were significant positive correlations between miRNA34b, miRNA-429, and follicle-stimulating hormone(r=0.466, P=0.001;
r=0.375, P=0.009, respectively) and significant negative correlations between miRNA34b, miRNA-181a, and Johnsen’s
score (r=-0.287, P=0.048; r=-0.351, P=0.015, respectively). There was statistically significant negative correlation between
miRNA-429 and testosterone (r=-0.330, P=0.022).
Conclusion: The expression levels of miRNA 181aandmiRNA-429 only were upregulated in NOA patients compared with
controls. Thus, they may represent useful noninvasive biomarkers for NOA.